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Moreover, surfactant inclusion has been reported to lower ROS levels and protect mitochondrial function by preserving ATP production in lung alveolar cells 76 , which aligns with our findings where its presence significantly reduced IL-8 gene expression and oxidative stress markers following CS exposure

Stopping typically leads to gradual return toward baseline

Peripheral blood mononuclear cells (PBMCs) from healthy donors, obtained from peripheral blood or buffy coats, were used as control

Availability of data and materials Consent forms and trial data will be available upon request from the Sponsor

The control group was fed a basal diet, with each chicken receiving 100 g of feed daily, under 16 h of light, with drinking water provided ad libitum