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Animals were housed two animals per cage and were weighed daily, and if cuprizone-fed animals weighed less than 85% of their body weight at the start of the experimental period, they were weighed twice daily and their feed was supplemented with normal powdered chow until they returned to above the 85% threshold

For oxidized glutathione measurement, an aliquot of the supernatant was incubated 60 minutes at room temperature with 10 mM 2-vinylpyridine (2-VP) to scavenge reduced glutathione

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The retrieved records, screened using Covidence, included English-language studies published between 2020 and 2025 that involved participants without significant comorbidities

The main lipotropics in a lipo B12 injection are inositol, choline, and methionine, each of which performs a critical role in the body