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Iron is tightly controlled at the systemic and cellular levels to prevent iron overload and toxicity, which is associated with liver disease, diabetes, osteoporosis, heart disease, atherosclerosis, and neurodegenerative diseases (Galaris et al., 2019

Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)