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On the other hand, in hypoxia, PDHs and VHL are inactivated by mild oxidative stress, and HIF-1 is released from PHDs and VHL, transferred into nuclei, where HIF-1 forms a heterodimer with HIF-1, and results in the transcription of hypoxia response elements (HREs) on DNA

The upper layer was collected, and absorbance was recorded at 520 nm using toluene as a blank

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Gently shake to ensure the complete the solubilization, and then administer the solution thus obtained by slow intravenous route.

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