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Although the precise receptor for SHBG has not been identified to our knowledge, SHBG has been reported to bind to G protein-coupled receptor 6A [194], which is proposed to regulate energy metabolism in insulin-sensitive tissues as well as stimulate the release of glucagon-like peptide-1 and adiponectin, key regulators of glucose homeostasis [195]

Proteins were visualized via chemiluminescence using the ECL Advance Western Blotting Detection Kit (Amersham Biosciences), and images were captured and analyzed by densitometry using a Kodak ID Version 3.6 Image Analyzer (Kodak, NY, United States)

For instance, IL-17 plays a critical role in granulocyte colony-stimulating factor (G-CSF) expression and neutrophil homeostasis, while IL-5 secreted by ILC2s contributes to maintaining antiviral immune homeostasis (61, 62)