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Conclusions Despite advancements in our understanding of DN, addressing the growing healthcare burden posed by this complication of diabetes remains challenging

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

The estimated pravastatin dose of 40 mg/kg body weight/day is based on the average drink consumption rate of 3.5 mL/day