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The samples were incubated on ice and centrifuged to separate the serum, which was then collected, transferred to clean tubes, and stored until further experimentation took place [2]

Because energy expenditure is increased within fat cells, the rate of cellular metabolism within these fat cells go up

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Imaging was performed using a Bio-Rad Chemidoc MP imaging station (Bio-Rad, Hercules, CA, USA)

The binding of AdoCbl to the full-length protein results in a decrease in DGC activity compared to the apo protein