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doi: 10.1038/s41467-019-10741-9

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Fatty acid synthase (FASN)-mediated FAS contributes to the proliferation and maturation of Tregs, and FAO provides the energy crucial for Treg infiltration into the TME

Transient DNA transfections of HEK293T cells were carried out in p35 dishes (uncoated Nunclon multidish six-well plates) using GeneCellin (BioCellChallenge, Signes, France) according to the manufacturers protocol