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The increase in glutathione was measured most robustly in the bloodstream, not the brain, where it is difficult to measure

The experimental group of mice was placed in a sleep deprivation device, stay up 20 h a day, rest 4 h, for 4 days, then rest 2 days, according to this schedule for four consecutive times

Additionally, a MitoSox-based cytometric analysis was employed to detect mitochondrial ROS formation in RTCs