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Cells were grown to 100% confiuency before use

This extension of gap bonds during in vitro maturation in the presence of PDE4 inhibitors could allow passage of metabolites, ions, nucleotides and amino acids, which improve oocyte cytoplasm maturation, bringing it near synchronization of nuclear and cytoplasmic maturation, which would favor blastocyst production and quality

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161 SatohT.KosakaK.ItohK.KobayashiA.YamamotoM.ShimojoY.et al (2008)

The results showed that both RFP-Lc3 + and AO + cells were dramatically increased in the zebrafish midbrains after sulindac treatment (Fig