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Raw files from LC-MS/MS analyses for cell pellets were submitted to MaxQuant software (ver 1.6.17.0, ver 2.0.3.0) for protein identification and label-free quantification with parameters: Carbamidomethyl (C) as a fixed modification and protein N-acetylation and methionine oxidation as variable modifications with a first search error window of 20 ppm and main search error of 6 ppm

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Chronically activated microglia in ALS gradually lose their immune functions and develop unconventional proteome

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Moreover, the TFR1, FPN, and DMT1 transcriptions are controlled by HIF via binding to their hypoxia-responsive elements, serving as a potent transcription factor