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Cells were fixed with 4% PFA, permeabilized with PBS (0.5% Triton-X) and stained overnight at 4 C using the serum of a CL patient (SE6209/13) with high antibody titer against L

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Second, we found that in post-infusion BM samples, CAR T cells of LTR have increased mTOR-pS6, pAKT, AMPK and LDHA expression, supporting metabolic plasticity, and effector immunometabolic profile compared to those of STR as well as a microenvironmental milieu promoting mTOR activity (Fig

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