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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

For example, silymarin, an antihepatotoxic agent, is a widely accepted drug for the treatment of liver diseases (6)

Environ Res 103:238246 Gagn F, Andr C, Blaise C, Pellerin J, Sherry J, Talbot A (2009) An investigation of the disruptive effect of pollution in cold- and warm-adapted clam populations

In addition, elevated ferritin may reflect hepatic steatosis and excessive iron accumulation