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Switching of modA expression from OFF to ON state within the middle ear produces a phenotypic switch in several genes during infection that increases disease severity and permits bacterial persistence by increasing cell adhesion, invasion and robust biofilm formation ( ModA16 ON state, NTHi upregulates multiple iron-acquisition factors including an iron ABC transporter substrate-binding protein, and haem/haemopexin-binding proteins HxuC and HxuB (Tram et al., 2024)

Front Endocrinol (Lausanne) 13:886451

EAAC1 gene expression is promoted by nuclear factor erythroid 2-related factor 2 (Nrf2) [64], regulatory factor X1 (RFX1) [65], and all-trans-retinoic acid (ATRA) [66], while miR-96-5p, which is one of the microRNAs (miRNAs), has a target sequence in the 3-UTR of EAAC1 and suppresses the protein expression of EAAC1 posttranslationally, leading to decreased GSH levels in the brain [67,68] (Figure 3)

For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

An example pathway is a supplement protocol for poor ovarian responders that could include antioxidant support and targeted GHK-Cu administration to the ovary in animal models before translating to human trials