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Once inside the CNS or upon receiving peripheral signals, resident microglia transform into a pro-inflammatory M1 phenotype, releasing damaging mediators such as ROS and cytokines [303, 318, 319]

Patch testing proves essential before applying new peptide eye products given high sensitivity potential - apply small amount to inner forearm for 24-48 hours watching for redness, itching, or irritation, then test on less sensitive facial area (cheek) for another 24 hours before progressing to eye area