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Next, the membranes were incubated with Alexa Fluor 488 secondary antibody in PBS-C/M containing 16.2 M Hoechst 33342 (Thermo Fisher Scientific) for 30 min at room temperature

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The following year, Burnett launched the Marlboro Man campaign, a series of ads featuring rugged cowboys and other macho men such as hunters and blue-collar types smoking Marlboros, and by the end of 1955 Marlboro sales had increased more than 3,000 percent

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