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500ng of total genomic DNA in 20l was denatured at 95C for 5 min, rapidly cooled down on ice, digested to single nucleotides by 20U of Nuclease P1 (NEB) at 37C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB) for 30 min at 37C

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2023 Aug 10;389(6):51426