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Aside from hypoxia, 2,3-DPG was proposed to have an anti-platelet aggregation function

Metabolites were identified and quantified using El-MAVEN (v0.12.1-beta) with metabolite retention times empirically determined in-house

BLI assays BLI measurements were performed using the Octet R4 platform (Sartorius Bioanalytical Instruments) with Octet BLI Discovery version 13.0

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To further substantiate this mechanistic link, we performed genetic ablation of TFRC/CD71 via CRISPR-Cas9 in four different CLL cell lines