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Bacillus , Pseudomonas , Enterobacter , Sphingomonas , Pantomonas , and Methylobacterium are the main bacterial genera involved in the fermentation of CTLs (Huang et al

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PCR reactions were prepared with 5 l of Quanta PerfeCTa One-Step SYBR Green Mix (Gaithersburg, MD), 2ul 5pmol primers, 50 ng RNA template, 0.1 l M-MLV reverse transcriptase (Promega, Madison, WI), and nuclease-free water for a 10 l total reaction volume