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During the high season (June-August), we add additional departures

The marrow was flushed with RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, or ImmunoCult-SF Macrophage Medium (Stem Cell Technologies) with 20 ng/mL murine M-CSF (PeproTech) to promote differentiation into macrophages

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Standard prenatal vitamins containing 2.5-10 micrograms may be insufficient for those with dietary deficiency, so dedicated B12 supplementation is recommended

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