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Subsequently, 100 l of BacTiter-Glo reagent was added to each well and the plate was shaken on an orbital shaker for 5 min before luminescence was measured

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It may be prudent to run these tests sooner and more often if a patient is experiencing symptoms of hypothyroidism, hyperthyroidism, or both

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Western Blot HepG2 cells were homogenized on ice with RIPA buffer supplemented with phosphatase and protease inhibitors (Pierce, Thermo Scientific, MA, USA)