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The tetO-Gls-t2a-mCherry transgenic mice were generated by cyagen who microinjected the pMM400sfi vector containing a codon optimized Gls-t2a-mCherry cassette

One possible explanation is that activation of STAT3 was not required because pretreatment with HNG or SHLP3 preconditioned the cells preventing the deleterious effects of gentamicin