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Furthermore, we focused on the marked differences between normal and disease conditions during the growth process (Figures 3, 4) and suggested that the metabolite variety was affected by DPSC treatment during disease progression

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Tsuchiya et al [2] used a 5 min centrifugation for this step

The capacity of NLCs for EO loading is greater than that of LS and SLNs, although the use of surfactants in the preparation process is inevitable