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To construct ENPP2 luciferase reporters, ENPP2 wild-type 3UTR and mutant 3UTR were inserted separately in multiple cloning regions of psiCHECK -2 Vector (Promega, Madison, WI, USA): psiCHECK-ENPP2-WT and psiCHECK-ENPP2-MUT

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The skin and the BBB represent two sequential barriers

Ipamorelin is a short-acting secretagogue targeting stomach ghrelin release without increasing appetite, lasting only hours

For a 10 mg semaglutide vial, most researchers add 2 mL of bacteriostatic water to achieve a 5 mg/mL concentration based on established protocols