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In the 1990s and early 2000s, the exact binding site for IgG on FcRn and the 1:2 stoichiometry of the interaction were then revealed through the structural studies by Bjorkman and colleagues [24, 34,35,36, 97, 98]

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For the final sterilization step, the chamber was closed, and the temperature and pressure were raised to 121 C and 0.103 MPa, respectively, for 20 min

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