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The nanomaterial suspension was ultrasonicated for 30 min in the dark, after which zeta potential, UV-visible absorption spectra, and particle size distribution were calculated using a Xetasizer Nano ZS90 (Malvern Instruments, Marburg, UK) [6]

If you are collecting samples for a 24 hour period, be sure to collect the first urine after you wake up the next day, too

Measurements for cell viability can be used to correlate cell behavior with the number of viable cells in a population, optimize the growth conditions of cell populations maintained in culture, test toxicology, or assess the efficacy of potential drug candidates

Fang ZM, Zhang SM, Luo H, Jiang DS, Huo B, Zhong X

coli BL21 (DE3) cells were induced with 0.04 mM IPTG at 25 C for 3 h