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Hits advance to PK/brain-exposure and HED anchoring to inform first-in-human dose-finding, thereby linking mechanism discovery to formulation design with translational checkpoints

When ER stress occurs, accumulated unfolded proteins recruit GRP78 and drive its dissociation from IRE1, PERK, and ATF6

Methods: We leveraged a dataset comprising 39 features and compared three feature selection methods: ANOVA, recursive feature elimination (RFE), and Lasso regression

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The method involved intracellular oxidation of non-fluorescent dye dihydrorhodamine-(DHR)-123 to the fluorescent rhodamine-123 (R123) caused by free radicals and hydrogen peroxide