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Samples in Laemmli buffer were boiled at 95 C for five min and applied to a 15% SDS gel, separated by SDS-PAGE, and stained with coomassie

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Statistical analysis All statistical analyses were performed with GraphPad Prism version 9.0.0 (GraphPad Software Inc., San Diego, CA, USA)

Calorie restriction: When following a rigid detox programme, you often cut out high-calorie processed foods and snacks, which is the real reason for any potential initial weight loss

But the removed portion contains a large number of parietal cells, which means acid and carrier protein production both take a significant hit