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When mice were treated with B-FAHF-2 or BBR at the equivalent concentration, anaphylactic protection was lost and pharmacokinetics showed the maximal plasma concentration (Cmax) of B-FAHF-2 was 289.30 185.40 ng/mL and BBR, alone, showed very low bioavailability with Cmax value of 35.13 47.90 ng/mL [44]

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Serum creatinine and blood urea nitrogen (BUN) assay Serum creatinine was determined using the Point creatinine assay (#C7548) according to the manufacturers directions

Future perspective With multiple proposed mechanisms for analgesic effect, future studies should focus on larger scale, randomized controlled trials to further describe both efficacy and safety in the acute perioperative patient