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Conversely, when stimulated with 100 mM caffeine, flexor digitorum brevis myofibers from SELENON deficient mice had reduced amplitude of Ca 2+ release compared to wild-type mice which suggests that the ryanodine receptor of the SR may be impaired with loss of SELENON ( 2+ from the SR so, although it is evident that SELENON loss results in impairment of Ca 2+ homeostasis, the origin of this defect remains unclear and may involve both the release (ryanodine receptor) and re-uptake (SERCA) of Ca 2+

The presence of astrocytes in cultures was assessed through quantification of the expression of Glial Fibrillary Acidic Protein (GFAP) [1,250, G3893]

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