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Description

Immunoprecipitation Cell lysates were precleared by incubation with Pierce TM Protein G Magnetic Beads (Thermo Scientific TM , USA) for 30 min on a rotator at 4 C

Ind J Exp Biol, 42: 1213-1232, 2002

Biomedicines 9

HT-1080 p21 KO : DMSO, 0.6 10 5 , Nutlin-3, 1.5 10 5

cell rounding and membrane blebbing reduced), and partially preserves mitochondrial membrane potential (JC-1 ratio: APAP 0.41 0.05 vs GHK-Cu+APAP 0.61 0.06