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On the subsequent day, the cells underwent three washes with PBST before incubation with secondary antibodies for 60 minutes

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In another study, it was demonstrated that mTOR/Akt pathway regulates the mitochondrial respiratory activities and the expression of complex I, II and IV of the electron transport chain trough 4E-BP1 (109)

Pharmacol Res 119:208218 Panahi Y, Khalili N, Hosseini MS, Abbasinazari M, Sahebkar A (2014) Lipid-modifying effects of adjunctive therapy with curcuminoidspiperine combination in patients with metabolic syndrome: results of a randomized controlled trial