cysteine taken with glutathione supplements Best NAC Supplement Metabolic Maintenance® NAC

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All cell lines were cultivated in complete growth medium(DMEM high glucose GlutaMAXTM Pyruvate, 10% (v/v) fetal bovine serum, 0.1 mM MEM NEAA, 25mM HEPES, puromycin 10 pg/mL, 100 U/ml Penicillin, 100 pg/ml Streptomycin) to 90 % confluency at 37C / 5 % C02 / 90 % humidity in a humidified C02 incubator and were split twice a week

Wei X, Roettger Y, Tan B, He Y, Dodel R, Hampel H, Wei G, Haney J, Gu H, Johnstone BH, Liu J, Farlow MR, Du Y (2012) Human anti-prion antibodies block prion peptide fibril formation and neurotoxicity

Then, proteins were diluted in ABC (100 mM)