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To performed taxonomic classification, we utilized Kraken2 program 5 and matched the sequences against the Kraken2-microbial database (September 2018, 30GB) (downloaded from This database contained genomic sequences from archaea, bacteria, fungi, protozoa, viral and Univec_Core

9101, RRID_AB:331646)

These responses can range from the release of hormones to the activation of enzymes or proteins

Although the successful application of TyU19 and KYV-201 in IMIDs encourages the further exploration of allogeneic CAR-T therapy in patients with severe and refractory IMIDs (120), the optimal timing for allogeneic CAR-T therapy in autoimmunity requires further investigation due to the potential unknown short- and long-term safety risks and toxicity associated with off-the-shelf CAR-T therapies (150152)

Importantly, we demonstrated that pre-activation of the VQ-precursor by a 4-h incubation with methyl vinyl ketone (MVK) enables rapid modification, even when the reaction time is shortened to 10 min (Supplementary Fig