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Methods Animal preparation The animal care and experimental protocols conformed to the recommendations in the 8th Edition of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH, revised 2011) and were in accordance with the Animal Management Rules of China (Documentation 55, 2001, Ministry of Health, China)

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Reconstitution Steps Pull the chosen volume of bacteriostatic water ( 2.0 mL for 5 mg/mL, or 1.0 mL for 10 mg/mL) into a sterile syringe