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The input/flowthrough and immunoprecipitation samples were prepared with 4X Laemmli buffer containing 10% Beta-Mercaptoethanol and then boiled at 85 C for 10 min

R., Chaturvedi S., GranadosFuentes D., and Herzog E

Branched-chain amino acids: catabolism in skeletal muscle and implications for muscle and Whole-body metabolism

in 2015, thanks to a study where supplementation with an anti-inflammatory molecule, the conjugated linoleic acid, led to a reduction in the expression of pro-inflammatory cytokines such as TNF- and IL-1 and metabolic dysfunction in offspring [76,79]

One way it does this is by increasing gene expression of the beneficial fat-related cytokine adiponectin (Tsuda 2004)