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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

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Small-molecule administration After shaving the backs of 7-week-old C57 mice, 100 l of 400 M KYP-2047 (T8657, Taojiao, China), 8 mM SMER28 (T3155, Taojiao, China), 200 M Pennogenin 3-O-beta-chacotrioside (T8166, Taojiao, China), or dimethyl sulfoxide (BS087-100 ml, Biosharp, China) was applied to the lower left part of the back of the mice