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Plasma lipids were extracted and the phospholipids were separated as previously described.21-22 The PC fraction was subjected to fatty acid analysis by capillary gas chromatography, and fatty acid content was quantified by digital integration as previously described,23 with the coefficients of variation being below 10% within and between runs

The detection limit was 0.094 ng/mL

Andersen J, Urban N, Achimastou A, Ito A, Simic M, Ullom K, et al

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At the 12-month follow-up : 87.5% of patients reported significant relief defined as 50% reduction in baseline pain scores Mean VAS pain score dropped from 7.2 at baseline to 2.8 at 12 months KOOS (Knee Injury and Osteoarthritis Outcome Score) improved across all subscales: pain, symptoms, activities of daily living, sport/recreation, and quality of life No serious adverse events were reported across the treatment period The durability of the response at 12 months is particularly notable