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NF-B exists in the cytoplasm in an inactive state, in a complex with the specific reaction inhibitor IB (IKK)

Self-amplifying RNAs generated with the modified nucleotides 5-methylcytidine and 5-methyluridine mediate strong expression and immunogenicity in vivo

PLoS One 8 (6), e67297

Total protein extracts (2040 g) were resolved by SDSPAGE under reducing conditions and transferred to polyvinylidene difluoride (PVDF) membranes, which were blocked with 5% non-fat milk or bovine serum albumin (BSA) in Tris-buffered saline with Tween-20 (TBST) to minimize nonspecific binding of primary antibodies, including rabbit polyclonal anti-FAM134B (1:1000

Responses of marine animals to ocean acidification