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Description

2.5 l of the sample was subjected to pre-column derivatization with OPA reagent (Agilent, 50613335) by programming the auto-sampler and the separation of amino acids was carried out in Agilent 1260 Infinity HPLC System (Agilent Technologies) using a Poroshell 120 HPH-C18 column (4.6 mm 100 mm X 2.7 m) as per the manufacturers protocol 70