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Once confluent, the media was aspirated, cells were washed with 1X PBS, and incubated with 2 mL 0 or 10 mM NAC for 2 h

DMEM/F12 (1:1) and FBS were gained from Gibco (Waltham, MA, USA)

Allow lyophilised peptide to reach room temperature before adding solvent to minimise shock-induced degradation

Fractions corresponding to the front, middle, and back parts of the Ub4 peak were collected separately

Findings from a cross-sectional analysis involving 450 adults diagnosed with MASLD revealed that greater adherence to the MIND diet was correlated with improved liver function, reduced systemic inflammation, enhanced metabolic health, as well as more favorable indicators of gut microbiome composition in MASLD patients