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doi: 10.3389/fnut.2022.958563 [DOI] [PMC free article] [PubMed] [Google Scholar] 142.Yan J, Chen S, Yi Z, et al

Introduction B

MolBlock representations were generated from SMILES 16 strings and optimized using the universal force field algorithm 17 to ensure chemically valid conformations

Glutathione levels naturally decline with age due to stress, toxins, illness, and ageing

For PCR amplification, the hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified using a forward primer (5-ACTCCTACGGGAGGCAGCAG-3) and a cocktail of four equally mixed reverse primers, including R1 (5-TACCRGGGTHTCTAATCC-3), R2 (5-TACCAGAGTATCTAATTC-3), R3 (5-CTACDSRGGTMTCTAATC-3) and R4 (5-TACNVGGGTATCTAATCC-3) 54