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Remaining amino acids were measured by mixing 10 L of the aqueous extract with 90 L 13 C- and 15 N-labeled amino acid standard solution (10 g per mL) and analyzed using an Agilent 1260 HPLC coupled to an API6500 tandem mass spectrometer

In addition, homocysteine is recycled to methionine through BHMT

IRB and IACUC Protocols: Researchers conducting animal model studies with peptides are required to obtain appropriate Institutional Review Board (IRB) or Institutional Animal Care and Use Committee (IACUC) approvals