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Various studies have evaluated clinical factors, biomarkers, radiological and physiological parameters (respiratory function tests and exercise capacity) and presence of comorbidities, associated with a higher risk of mortality, both at the time of follow-up and developmentally during follow-up.28 Factors associated with poor evolution are (Table 4): - Aged over 70 years.28 - Associated comorbidities: pulmonary hypertension, emphysema and bronchogenic carcinoma

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Duarte JM, Do KQ, Gruetter R (2014) Longitudinal neurochemical modifications in the aging mouse brain measured in vivo by 1 H magnetic resonance spectroscopy

Polyhistidine-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture