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Preclinical studies using T4 (including the RGN-259 ophthalmic clinical trials) have not identified significant adverse events at therapeutic doses

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The authors aimed to evaluate the clinical efficacy of prolotherapy with 5% dextrose in water (D5W) injected into the multifidus muscle, versus mechanical needling combined with sterile water injection administered into multiple posterior lumbar structures, including facet joints, medial branches, and multifidus muscle [63]