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Prevention of chronic diseases by tea: possible mechanisms and human relevance

This was done by enrichment analysis of the most comprehensive model of mammalian metabolism, the Recon 2 metabolic network 41

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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI