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Plasma from healthy and critically ill patients was diluted to 4 mg/ml and treated with 10 mM DNPH for 45 min at RT

Other alternative therapies Below are a couple of alternative therapies with limited supporting evidence that pose significant risks

Frommer L, Kahaly GJ

RLS is associated with MCAS and histamine intolerance

The implementation of a small-volume, whole blood stimulation assay for the assessment of vaccine antigen-specific responses that can be set up at the trial site using minimal equipment, followed by sample preservation in RNA stabilisers and storage or transfer to the investigating laboratory for downstream processing, facilitates immunogenicity testing in clinical and field trials