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In contrast, knockdown of RRM2 significantly inhibited the migration and invasion ability of THCA cells compared with the control group ( Figure 7C , Supplementary Figure S6 )

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To test this, we first assessed cytoplasmic free mtDNA levels under hypoxia using immunofluorescence, and M9a+sh EPAS1 cells exhibited significantly reduced cytoplasmic free mtDNA compared to both M9a+shCTR and M7/8+shCTR cells (Fig