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Pachymic acid, a competing activator of pyruvate kinase M2, mimics fructose-1,6-bisphosphate to blockage glycolysis and lactate production

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Here, we use a pooled, dual CRISPR screening strategy that knocks out pairs of transporters in four metabolic states glucose, galactose, OXPHOS inhibition, and absence of pyruvate designed to unmask the inter-dependence of these genes

Samples were completely lysed using an ultrasonic homogenizer in an ice bath for 20 min and centrifuged at 14,000 rpm under 4C for 10 min