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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

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Dubreuil JD (2008) Escherichia coli STb toxin and colibacillosis: knowing is half the battle

doi: 10.1038/s41374-020-00492-3